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KMID : 0616120120430040316
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 4 p.316 ~ p.322
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Involvement of Heme Oxygenase-1 Induction in the Neuroprotective Activitiy of Extract of Siegesbeckia Herba in Murine Hippocampal HT22 Cells
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Im Nam-Kyung
Lee Dong-Sung
Yeo Sun-Jung
Kim Youn-Chul
Jeong Gil-Saeng
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Abstract
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Siegesbeckia Herba is known to have anti-oxidant, anti-inflammatory, anti-allergic and anti-tumor. The objective of this study is to explore the neuroprotective effect of Siegesbeckia Herba against glutamate-induced oxidative stress in mouse hippocampal HT22 cells. Siegesbeckia Herba 70% ethanol extract and solvent fractions have the potent neroprotective effects on glutamate-induced nerotoxicity by induced the expression of heme oxygenase (HO)-1 in the mouse hippocampal HT22 cells. Especially, ethyl acetate fraction showed higher protective effect. In HT22 cell, Siegesbeckia Herba ethyl acetate fraction makes the nuclear accumulation of Nrf2. Further, we found that treatment with c-JUN N-terminal kinase (JNK) inhibitor (SP600125) reduced Siegesbeckia Herba ethyl acetate fraction induced HO-1 expression and Siegesbeckia Herba ethyl acetate fraction also increased JNK phosphorylation. In conclusion, the ethyl acetate fraction of 70% ethanol extract of Siegesbeckia Herba significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2 and JNK pathway in mouse hippocampal HT22. Taken together these finding suggest that Siegesbeckia Herba ethyl acetate fraction good source for taking active compounds and may be a potential therapeutic for brain disorder by targeting the oxidative stress of neuronal cell.
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KEYWORD
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Siegesbeckia Herba , ROS , Heme oxygenase-1 , HT22 , Glutamate , Neroprotective effect
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